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This Concept Map, created with IHMC CmapTools, has information related to: LAB 16 - SEROLOGY, PART 1 - DIRECT SEROLOGIC TESTING, plasma definition The liquid fraction of the blood before clotting., serological typing of Shigella description Mix the suspected Shigella, the unknown antigen, with know subgroup A, B, C, and D, antiserum. Mix and look for agglutination of the bacteria., serology definitions immunogen, direct serologic testing examples using the direct fluorescent antibody technique to identify microorganisms, serology definitions antigen, antigen chemical nature large molecular weight proteins and polysaccharides, direct serologic testing concept Antigen-antibody reactions are very specific. Antibodies usually react only with the antigen that stimulated their production in the first place., epitope definition The actual portions of an antigen that react with receptors on B-lymphocytes and T-lymphocytes, as well as with free antibody molecules; 5-15 amino acids, 3-4 sugar residues., Used to identify unknown antigens (such as microorganisms). A preparation known antibodies, called antiserum, is used to identify an unknown antigen. antiserum is prepared by: animal inoculation with a specific antigen, using the direct fluorescent antibody technique to identify microorganisms description Heat-fix suspected Neisseria gonorrhoeae, the unknown antigen, to a microscope slide. Add known fluorescent antibody against N. gonorrhoeae, wash, and view under a fluorescent microscope. Look for fluorescing gonococci., serology types direct serologic testing, direct serologic testing examples serological typing of Streptococci, direct serologic testing examples serological typing of Shigella, direct serologic testing description Used to identify unknown antigens (such as microorganisms). A preparation known antibodies, called antiserum, is used to identify an unknown antigen., large molecular weight proteins and polysaccharides examples microbial structures, microbial exotoxins, allergens, foreign tissues and cells, the body's own cells that the body fails to recognize as "normal self", serological typing of Streptococci description Lyse the suspected group A beta streptococcus, the unknown antigen. Place the test strip with known anti-Strep A antibody-red latex conjugate in the lysed bacteria, and look for the formation of two red bands., serology definitions plasma, serological testing to diagnose pregnancy description Place the test strip with known anti-hCG antibody-red latex conjugate in the urine, and look for the formation of two red bands., Used to identify unknown antigens (such as microorganisms). A preparation known antibodies, called antiserum, is used to identify an unknown antigen. antiserum is prepared by: monoclonal antibody technique, humoral immunity definition The production of antibody molecules in response to an antigen; mediated by B-lymphocytes.